许晓燕; 郑林用; 李艳; 江南; 余梦瑶; 罗霞
四川省中医药科学院中药细胞与分子生物学实验室; 四川省农业科学院; 成都市经济信息中心

【中文摘要】 采用双向固体发酵工程技术,以灵芝为发酵菌株,在含有不同比例丹参边角料的培养基中进行发酵,观察了不同培养基中菌丝的生长情况,测定了发酵产物(灵丹菌质)中多糖、蛋白质、三萜、丹参酮的含量变化,并研究了其提取物对小鼠活血化瘀功效的影响。结果表明灵丹菌质的最佳发酵组合为组合4,发酵40d,多糖含量26.48μg·g-1,蛋白质含量为397.24μg·g-1,灵芝酸三萜含量为4.92%。药效学实验显示,灵丹菌质组合4具有活血化瘀的药效,优于丹参药材。

【英文摘要】 With two-way pattern solid-state fermentation technique, Ganoderma lucidum was fermented in the drug-containing medium containing different proportion of Salvia miltiorrhiza bunge by-product. Hyphal growth of G. lucidum was surveyed. The contents of polysaccharide, water-solubility protein, triterpene and tanshinton of solid-fermented products (SFP) were tested. In addition, the effect of activiting blood circulation to dissipate blood stasis on mouse fed with extracts was evaluated. The results showed that formulation 4 was the optimal medium, of which fermentation period was 40 d. Polysaccharide, crude protein, Ganoderma tanshinton of optimal SFP were 26.48 mg·g-1, 397.24 μg·g-1and 4.92%, respectively. From the results of pharmaco-dynamical experiments, the effects of SFP were better than that of Salvia miltiorrhiza.

【中文关键词】 灵芝; 丹参边角料; 双向发酵; 药效品质
【英文关键词】 Ganoderma lucidum; Salvia miltiorrhiza bunge by-product; Two-way pattern solid-state fermentation; Pharmacody- namic action
【基金】省中医管理局项目;; 省应用基础项目(2008JY0006-2)

【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2010年02期 【DOI】CNKI:SUN:ZSYJ.0.2010-02-013