操玉平; 于敏; 沈业寿; 陆军; 许宝奎
安徽大学生命科学学院安徽大学中药研究与开发重点实验室

【中文摘要】 培养大鼠胰岛素瘤细胞(INS-1),以四氧嘧啶(AXN)损伤细胞,培养液中加入不同浓度的多糖AMP-1,检测不同浓度AMP-1对INS-1细胞葡萄糖刺激的胰岛素和C肽分泌量的影响,同时检测细胞存活率。结果表明,一定浓度范围AMP-1对AXN损伤的INS-1细胞分泌胰岛素和C肽均具有一定的促进作用,尤其是在葡萄糖刺激浓度为16.7mmol·L-1时,效果显著;AMP-1可减少AXN对INS-1细胞的损伤,使INS-1细胞存活率增加。

【英文摘要】 Graded concentrations of AMP-1 were added into the culture medium of INS-1 cells injured by alloxan. The amount of glucose-stimulated insulin and C-peptide secretions in different concentration of AMP-1 was determined and the survival rate was measured. The results showed that graded concentrations of AMP-1 could promote glucose-stimulated insulin and C-peptide secretions in INS-1 cells, especially in the concentration of 16.7 mmol·L -1 of glucose. AMP -1 could reduce the injuries induced by alloxan in INS-1 cells and the survival rate of cells increased significantly.

【中文关键词】 蜜环菌; 菌索多糖; 四氧嘧啶; 胰岛素瘤细胞; 胰岛素; C肽
【英文关键词】 Armillariella mellea; AMP; Alloxan; INS-1 cells; Insulin; C-peptide
【基金】国家自然科学基金项目(3040066);; 安徽省教育厅自然基金项目(2003kj021;2002kj017)。

【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2009年01期 【DOI】CNKI:SUN:ZSYJ.0.2009-01-019