虞磊; 沈业寿; 李赓; 吴建民; 谢群英
安徽大学生命科学学院糖复合物与糖工程实验室; 安徽省生态工程与生物技术重点实验室; 安徽大学生命科学学院糖复合物与糖工程实验室; 安徽大学生命科学学院糖复合物与糖工程实验室; 安徽大学生命科学学院糖复合物与糖工程实验室 合肥230039; 合肥230039; 合肥230039; 合肥230039; 合肥230039
【中文摘要】 对蜜环菌在不同液态培养方式下胞内多糖的合成、组成以及抗眩晕症活性进行比较。结果显示,蜜环菌在摇床培养条件下形成菌丝球,菌丝多糖(AMP)和生物量在第8d达到最高产量,分别为124.32mg/L和10.85g/L,蜜环菌在静置培养条件下形成菌索,菌索多糖(ARP)与生物量在20d时达到最高,分别为588.40mg/L和37.17g/L,静置培养对蜜环菌胞内多糖的合成有利;两种多糖在多糖含量、蛋白质含量、糖醛酸含量以及皂苷含量等组成成分上基本一致;AKTA-purifier快速层析纯化系统(FPLC)检测显示,AMP与ARP有四个相同的多糖组分,ARP另多出一个组分;抗机械旋转所致小鼠眩晕症试验表明AMP与ARP活性相似,均能显著缩短眩晕小鼠逃避电击所用的时间(P<0.01),增加小鼠眩晕后的进食量,因而对机械旋转所致眩晕症具有一定疗效。
【英文摘要】 To study the effects of intrapolysaccharides production and component from Armillaria mellea in different liquid culturing methods and anti-vertigo function.Results showed that,under submerged cultivation,AMP(polysaccharides from mycelium of Armillaria mellea) and biomass achieves the maximum production rate in 8th days,were 124.32mg/L and 10.85 g/L respectively.ARP(polysaccharides from rhizomorph of Armillaria mellea) and biomass reached 588.40mg/L and 37.17g/L,at the time of 20 days.Quiet cultivation is better of intrapolysaccharides production for Armillaria mellea.Two intrapolysaccharides have close composition in polysaccharide content,protein content,uronic acid content,and saponin content;FPLC showed that AMP and ARP have four similar polysaccharides components,ARP also have another component;The experiment of opposing function of vertigo caused by machinery rotate to mice showed that AMP and ARP can obviously shorten the escaping time of electrical shock(P<0.01),and obviousl...更多y increase food intake in mice,so the intrapolysaccharides of Armillaria mellea have anti-vertigo function. 还原
【中文关键词】 摇床培养; 静置培养; 胞内多糖; FPLC; 抗眩晕
【英文关键词】 submerged cultivation; quiet cultivation; intrapolysaccharides; FPLC; anti-vertigo
【基金】国家自然科学基金(3040066);; 安徽省教育厅自然科学基金(2003kj021)
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2006年02期 【DOI】CNKI:SUN:ZSYJ.0.2006-02-014