【作者】胡忠策 郑晓冬 陈新爱 郑裕国
【机构】College of Biosystem Engineering and Food Science Zhejiang UniversityCollege of Biosystem Engineering and Food Science Zhejiang UniversityInstitute of Bioengineering Zhejiang University of TechnologyHangzhou 310029 China Institute of Bioengineering Zhejiang University of Technology Hangzhou 310032 China
【摘要】A species of mushroom, Termitomyces albuminosus, was cultured in liquid medium for production of chromium-enriched mycelium. The influence of chromium (Ⅲ ) on mycelial growth of T. albuminosus was investigated. An optimum medium composed of 5.6g/L yeast extract, 51.6g/L hydrolyzed rice, 2g/L KH2PO4, and 20mg/L chromium(Ⅲ ) with initial pH of 4.5 was obtained by using method of central composite design (CCD). After incubation of 84h, the maximal biomass of chromium-enriched mycelia reached 24.23g DMW(dried mycelial weight)/L with 272μg/g DMW chromium content in 500mL flasks containing 100mL medium with an inoculum of 8% on a shaker of 100r/min under an optimized cultivation condition at 28℃.