黄达明; 刘芹; 张志才; 张玲; 仲媛; 赵继友
江苏大学食品与生物工程学院

【中文摘要】 探索树舌发酵产生高活性的碳酸酐酶抑制剂的营养需求。试验以抑制碳酸酐酶活性为指标,通过单因素试验筛选出适宜碳源、氮源及复合营养因子,通过正交试验进一步观察了各组分之间的相互作用。试验结果表明,树舌发酵合成碳酸酐酶抑制剂的较为适合的培养基为:果糖20 g.L-1、NaNO3 2 g.L-1、马铃薯173 g.L-1、(NH4)2SO4 1.3 g.L-1。使用该培养基其发酵液对碳酸酐酶的抑制率可达91.43%,显著高于未优化的培养基(69.81%)。

【英文摘要】 In this study,the nutritional needs of Ganoderma applanatum to produce high active carbonic anhydrase inhibitors was investigated.Firstly,optimal carbon and nitrogen sources,and natural components were selected by single factor experi-ments according to carbonic anhydrase inhibition ratio of the fermentation broth.Then,inter-action of these factors was observed by orthogonal testsstyle.Finally,the inhibitory ratio based on basal medium and optimized medium was compared.The opti-mized medium contained: fructose 20 g.L-1,NaNO3 2 g.L-1,potato 175 g.L-1,(NH4)2SO4 1.3 g.L-1,In the medium,the highest inhibition ratio(91.43%) was achieved which was significantly higher than basal medium(69.81%).

【中文关键词】 树舌; 碳酸酐酶抑制剂; 培养基优化
【英文关键词】 Ganoderma applanatum; Carbonic anhydrase inhibitors; Medium optimization
【基金】国家973基金项目(40721002);; 江苏安慧生物科技有限公司资助项目(项目编号2006278);; 江苏大学高级人才专项基金资助(项目编号1283000268)

【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2009年06期 【DOI】CNKI:SUN:ZSYJ.0.2009-06-016