【作者】延光海; 李良昌; 沈光海; 李光昭

【Author】YAN Guanghai1; LI Liangchang1; SHEN Guanghai2; LI Guangzhao11Department of Anatomy; Yanbian University School of Basic Medical Sciences; Yanji; Jilin 133000; China; 2 College of Pharmacy; Yanbian University; Yanji; Jilin 133000; China

【作者机构】延边大学医学部解剖学教研部; 延边大学医学部药学院

【摘要】 通过IgE诱导大鼠被动皮肤过敏反应(PCA)实验、细胞毒性试验、IgE诱导肥大细胞组胺释放、钙摄入量实验、Western blot试验,来研究桦褐孔菌(Inonotus oblipuus)乙醇提取物EEIO的抗过敏作用。试验发现,EEIO明显抑制了大鼠皮肤伊文思蓝渗出,对IgE诱导肥大细胞的细胞内钙摄入、组胺以及肿瘤坏死因子-α、白细胞介素6的释放有剂量依赖抑制作用。表明,桦褐孔菌乙醇提取物具有一定的抗过敏作用。

【Abstract】 We have studied the anti-allergic effects of an orally-administered ethanolic extract of Inonotus obliquus(EEIO) by evaluating anti-DNP IgE-mediated passive cutaneous anaphylaxis(PCA) in rats,EEIO cytotoxicity,histamine release,calcium uptake,and TNF-α and IL-6 secretion by IgE-induced rat peritoneal mast cells(RPMCs) incubated with EEIO.EEIO(1 100 μg/mL) had no adverse effect on RPMC viability under the test conditions,and significantly inhibited Evans blue leakage in antigen-challenged rats sensitized by anti-DNP IgE.EEIO also suppressed antigen-stimulated calcium uptake and histamine release in RPMCs,and Western blot analysis revealed that EEIO inhibited the antigen-stimulated expression of TNF-α and IL-6 in RPMCs in a dose-dependent manner.Our data indicate that EEIO exhibits anti-allergic effects.

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【Key words】 Inonotus obliquus; mast cell; IgE; histamine; calcium; TNF-α; IL-6

【基金】吉林省教育厅项目(编号:吉教科合字[2009]第28号)的部分研究内容~~

【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2010年02期

【DOI】CNKI:SUN:SYJB.0.2010-02-014

【分类号】R285.5