TI: Molecular systematics of the genus Pleurotus: analysis of restriction polymorphisms in ribosomal DNA.
AU: Iracabal-B; Zervakis-G; Labarere-J
SO: Microbiology-Reading. 1995, 141: 6, 1479-1490; 49 ref.
LA: English
AB: Part of the nuclear ribosomal DNA unit of Pleurotus cornucopiae, including most of the intergenic sequences, was used as a probe to hybridize with DNA from eleven Pleurotus taxa (29 isolates), digested with nine restriction endonucleases. For all digests, a high level of rDNA heterogeneity between and within species was detected, which is in agreement with the phenetic variability already reported in previous studies on Pleurotus taxa. Numerical analysis of the results, performed by use of two different tree-making methods, clearly distinguished among well-defined species, i.e. P. dryinus, P. eryngii and P. flabellatus, which presented large phenetic distances with the rest of the taxa tested. P. abalonus, despite morphological similarity and interfertility with P. cystidiosus, appears to be a discrete taxon. Although they showed relative affinity, P. cornucopiae, P. columbinus and P. ostreatus presented large distances among their representative isolates, supporting the idea that they correspond to distinct taxa. All strains of P. pulmonarius, P. sajor-caju and P. sapidus having small phenetic distances were closely positioned on dendrograms; in relation to results from recent interfertility and isozyme studies, these results support the common genetic background of P. pulmonarius and P. sajor-caju; the position of P. sapidus remains controversial. Furthermore, the rDNA analysis identified ten fragments suitable for species identification: eight allowed the characterization of five taxa (P. cornucopiae, P. ostreatus, P. sajor-caju, P. abalonus and P. columbinus) and two distinguished among clusters including related taxa. By a combination of just two restriction enzymes, the rDNA probe used seems to be the appropriate tool for both varietal characterization and protection of commercially valuable strains.
PT: Journal-article
AN: 961605736
TI: PCR-based restriction analysis of internal transcribed spacers of nuclear ribosomal DNA in the genus Pleurotus.
AU: Ma-FuYing; Luo-XinChang; Ma-FY; Luo-XC
SO: Mycosystema. 2002, 21: 3, 356-362; 9 ref.
LA: English
LS: Chinese
AB: The internal transcribed spacers of the nuclear ribosomal DNA of eighteen Pleurotus taxa (52 isolates), together with outgroups Agaricus bisporus, Lentinula edodes, and H. serotina, were amplified using PCR and then digested with seven restriction endonucleases. PCR-RFLP results showed that AluI, HaeIII, HinfI, TaqI, HhaI, and MspI divided 52 isolates into 6, 5, 5, 4, 2, and 2 groups, respectively, and distinguished P. citrinopileatus from the other Pleurotus spp. No restriction site was observed for BamHI in all isolates. Dendrogram based on PCR-RFLP suggested that seven groups were distinguishable for Pleurotus on 93% similarity coefficient, i.e. P. ostreatus complex (including P. ostreatus, P. florida, P. sapidus, P. corticatus, P. cornucopiae, P. columbinus, P. spodoleucus, P. ferulae, P. nebrodensis, and Pleurotus sp.), P. eryngii, P. pulmonarius, P. sajor-caju, P. tuber-regium, P. abalonus, P. cystiodisus, P. djamor, P. salmoneostramineus, and P. citrinopileatus. The phylogenetic relationships between P. citrinopileatus and the other Pleurotus spp. were more distant than those of L. edodes, A. bisporus, and the other Pleurotus spp.
PT: Journal-article
AN: 20023162004
