【作者】孙晓红; 陈明杰; 汪虹; 冯爱萍; 潘迎捷

【Author】SUN Xiaohong1; 2; 3; CHEN Mingjie1; WANG Hong1; FENG Aiping1; PAN Yingjie31National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization; Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi; Shanghai Academy of Agricultural Sciences; 201106; P. R. China; 2College of Life Sciences; Nanjing Agricultural University; Nanjing; Jiangsu 210095; China; 3Shanghai Ocean University; Shanghai 200090; China

【作者机构】国家食用菌工程技术研究中心; 农业部应用真菌资源与利用重点开放实验室; 上海市农业遗传育种重点开放实验室; 上海市农业科学院食用菌研究所; 南京农业大学生命技术学院; 上海海洋大学

【摘要】 根据草菇(Volvariella volvacea)S-腺苷-L-高半胱氨酸水解酶基因(SAHH)全长cDNA序列设计引物,以草菇基因组DNA为模板,PCR扩增获得草菇SAHH基因的全长序列,序列分析结果表明,草菇SAHH长度为1868bp,编码430个氨基酸残基,基因由12个外显子和11个内含子组成,将草菇SAHH基因推断的氨基酸序列与其它物种的SAHH基因氨基酸序列进行比较发现,SAHH基因保守性较高。

【Abstract】 The genomic sequence of a S-adenosyl-L-homocysteine hydrolase gene (SAHH) from Volvariella volvacea was amplified using specific primers designed according to the full-length SAHH cDNA. SAHH consists of 1 868 bp encoding 430 amino acids,and contains 12 exons and 11 introns. A phylogenetic tree constructed on the basis of the amino acid sequences of SAHH genes in V. volvacea and other reference species revealed a high degree of conservation.

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【Key words】 Volvariella volvacea; S-adenosyl-L-homocysteine hydrolase gene; clone; sequence analysis

【基金】农业部公益性行业科研专项项目(编号:nyhyzx07-008);; 上海市科技兴农重点攻关项目[编号:沪农科攻字(2007)第6-2号]的部分研究内容

【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2010年01期

【DOI】CNKI:SUN:SYJB.0.2010-01-006

【分类号】S646.13