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Construction of a Plasmid and a Standard Curve for Real-time Quantitative PCR of the Cold-induced Co


【发布日期】:2010-05-17

WANG Hong; CHEN MingjieKey Laboratory of Agricultural Genetics and Breeding of Shanghai; Institute of Edible Fungi; Shanghai Academy of Agricultural Sciences; Shanghai 201106; China

【中文摘要】 A plasmid containing target DNA and a standard curve for real-time quantitative PCR of the cold-induced Cor3 gene of Volvariella volvacea were constructed. These will provide the basis for further research on Cor3 gene expression at low temperature, and ultimately for assigning a role for the gene in the low temperature autolysis of V. volvacea.

【英文摘要】 A plasmid containing target DNA and a standard curve for real-time quantitative PCR of the cold-induced Cor3 gene of Volvariella volvacea were constructed. These will provide the basis for further research on Cor3 gene expression at low temperature, and ultimately for assigning a role for the gene in the low temperature autolysis of V. volvacea.

【英文关键词】 Volvariella volvacea; Cold induction; Cor3 gene; Quantitative PCR; Plasmid; Standard curve
【基金】Sponsored by the Shanghai Academy of Agricultural Science and Shanghai Science Committee (No .003912076)

【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2007年03期 【DOI】CNKI:SUN:SYJB.0.2007-03-006

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