【作者】 杨玉红； 刘芳； 康宗利； 段玉玺；

【Author】 YANG Yu-hong1,2,LIU Fang2,KANG Zong-li2,DUAN Yu-xi1(1.Plant protection Post-doctoral Flow Station in Shenyang Agriculture University,Shenyang Liaoning 100866; 2.Biological Science Technology Institute of Shenyang Agriculture University,Shenyang Liaoning 100866)

【机构】 沈阳农业大学植物保护博士后流动站； 沈阳农业大学生物科学技术学院；

【摘要】 随着生产规模的不断扩大,北虫草的培育也暴露了一个问题,正常菌种经多次转管继代和冰箱久存之后,会出现不产子实体现象,这些问题严重干扰了虫草的生产。为此,进行了有关北虫草不同部位组织分离和继代培养的试验,并筛选出性状优良的菌株。结果表明,子实体靠近顶端分离的菌种易于萌发,孢子分离得到的菌株性状更好,北虫草子实体本身就具有抑菌作用,因此,在选取表面消毒方式时,一定要适宜,不可时间过长,以免对子实体造成伤害,不产生菌丝。

【Abstract】 With the procreative scale dilating constantly,the culture of Cordyceps sinensis had some questions.when the normal bacterium was transferred many times or be kept in the fridge for a long time,it maybe didn't produce the fructification.So an experiment on the Cordyceps military tissue isolation and continuous culture were done,and a strong spices were got.The results showed that the part near top was more likely to sprout.North Cordyceps was some kind of bacteriostat.The one which isolated from...

Manbetx体育手机版推荐A9版本 北冬虫夏草； 人工栽培； 组织分离； 子实体；

【Key words】 Cordyceps sinensis； Artificial cultivation； Tissue isolation； Fructification；

【分类号】S646.9